Composite

Part:BBa_K1154006

Designed by: Adam Nighswander, Tanner Reeb and Bianca Maled   Group: iGEM13_RHIT   (2013-09-20)

Mating pheromone-induced IGPD and constitutive LDH expression in yeast

Synthetic sequence optimized for expression in Saccharomyces cerevisie. The construct harbors a yeast imidazoleglycerol-phosphate dehydratase coding sequence (IGPD) under control of the mating pheromone-induced FUS1 promoter. It also contains the coding sequence for human lactate dehydrogenase (LDHA) under control of the constitutive TEF2 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 763
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None